The second CAS Conference on Synthetic Biology took place in Munich from 27 to 29 July 2015. It is one of the largest conferences in this field in Europe. Top European scientists as well as excellent scientists from US were invited. The conference is a platform to exchange and discuss cutting-edge results on Synthetic Biology. The topics covered all aspects ranging from mirror biology, orthogonal biosystems, metabolic and genome engineering technologies as well as translational aspects of Synthetic Biology. Additionally, one session was on strategic European activities.